Fig. 8: SPOC domains determine genomic localization.

a Cellular localization of SPOC proteins analyzed by immunofluorescence microscopy. Exemplary Airyscan images showing the localization of wild-type and SPOC-deleted proteins for PHF3, DIDO, SHARP, and RBM15 (green). DAPI and merged images are shown below. Localization of each protein of interest was acquired in biological duplicates. Scale bar = 10 μm. b Integrative Genomics Viewer (IGV) snapshots showing GFP-ChIP-seq reads and GFP-ChIP-qPCR analysis for XIST in SHARP-GFP, SHARP ΔSPOC-GFP, and untagged HEK293T cells. c IGV snapshots showing RNA-seq reads for XIST in SHARP-GFP and SHARP ΔSPOC-GFP cells. d IGV snapshots showing GFP-ChIP-seq reads and GFP-ChIP-qPCR analysis for BEX5 in PHF3-GFP, PHF3 ΔSPOC-GFP, and untagged HEK293T cells. e IGV snapshots showing GFP-ChIP-seq reads and GFP-ChIP-qPCR analysis for HOXA5 in PHF3-GFP, PHF3 ΔSPOC-GFP, and untagged HEK293T cells. The qPCR data in b, d, e are presented as mean ± standard deviation of four individual experiments, individual data points are indicated as black dots. One-tailed, two-sample equal variance t-test was used to determine p-values. qPCR amplicons are indicated as red boxes. Source data are provided as a Source Data file.