Fig. 1: RING1B remains bound to target gene promoters across interphase.

a Heatmaps of normalized RING1B ChIP-seq reads around the TSS (±10 kb) of target promoters (n = 10,908) at different phases of the cell cycle. Genes are ranked according to the signal in G2 phase. b Average binding profile of RING1B around the TSS of RING1B target promoters in G1 (red), S (green) and G2 (blue) phases. c Correlation analysis between the signal of RING1B binding around the TSS (−0.5 kb to +1.5 kb) of target genes during G1 (x-axis) and G2 (y-axis). Linear regression R² and Pearson coefficient (r) are indicated. d Venn diagram showing the overlap between HC bivalent promoters (blue) and RING1B target promoters (orange). HC bivalent promoters were previously defined¹⁹. e Average binding profile of RING1B around the TSS of target genes that are HC bivalent promoters (left panel) or not (right panel) defined in (d) in G1 (red), S (green), and G2 (blue) phases. f Boxplots comparing 4sU-seq RNA reads (RPM) mapped to the proximal promoter region (TSS to +3Kb) of RING1B targets depending on whether they are HC bivalent or not in the indicated cell cycle phases. Genes that are not bound by RING1B (0.2 > Log₂ FC G1/G2 > −0.2) are shown as a control. Boxes show median and Q1–Q3 values. Whiskers denote the 1.5× the interquartile range. Mann–Whitney test was applied (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). g MA plot of fold change gene expression between cells in G1 and G2 phases (4sU-seq normalized reads mapping from the TSS to +3 kb) at HC bivalent RING1B targets (black dots) and Not HC bivalent RING1B targets (grey dots). Nascent RNA expression in G1 is represented in the x-axis. h Average 4sU-seq RNA reads at RING1B + HC bivalent promoters in G1 (red), S (green), and G2 (blue) phases. P indicates one-way ANOVA test p-value. i Genome browser view of RING1B binding and nascent RNA synthesis at indicated cell cycle phases at the bivalent gene Hoxd13. Source data are provided as a source data file.