Fig. 4: Developmentally regulated transcription factors are common targets of PRC2/cPRC1/vPRC1 that display enhanced recruitment of PRC1 during G2 compared to the G1 phase.
From: Changes in PRC1 activity during interphase modulate lineage transition in pluripotent cells
a Venn diagram showing the overlap between RING1B (blue), RYBP (yellow), CBX7 (green) and EZH2 (red) target promoters. Gene Ontology analyses of gene promoters bound by vPRC1, cPRC1 and PRC2 (n = 2093) or by vPRC1 only (n = 6222) are shown on the right. Fisher’s exact test was used to calculate the P values. b Average binding profile around the TSS of RING1B, RYBP, H2AK119ub1 and CBX7 at vPRC1/cPRC1/PRC2 (top row) or vPRC1 specific (bottom row) target genes (as defined in a) in G1 (red), S (green) and G2 (blue) phases. P indicates one-way ANOVA test p-value. c Boxplots comparing 4sU-seq nascent RNA reads (RPMs, TSS to +3Kb) of vPRC1/cPRC1/PRC2 targets (n = 2093) and vPRC1 specific targets (n = 6222) at indicated phases of the cell cycle. Boxes show median and Q1–Q3 values. Whiskers denote the 1.5× the interquartile range. Mann–Whitney test was applied (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). d Violin plots showing the fold change binding between G2 and G1 of RYBP, H2AK119ub1, CBX7 and EZH2 at promoter regions (−0.5 kb to +1.5 kb relative to TSS) of vPRC1/cPRC1/PRC2 (green) and vPRC1 specific target genes (grey). Asterisks (***) mark statistically significant differences (P < 0.001) using Mann–Whitney test. e MA plot showing fold change gene expression between cells in G1 and G2 phases (4sU-seq normalized reads mapping from the TSS to +3 kb) at vPRC1/cPRC1/PRC2 (green dots) and vPRC1 specific target promoters (grey dots). Binding of RING1B in G1 is represented in the x-axis. Percentage of up- or downregulated (above threshold FC > 1.2) genes for are indicated. Source data are provided as a source data file.
