Table 1 Data collection and refinement statistics
From: The unifying catalytic mechanism of the RING-between-RING E3 ubiquitin ligase family
| Â | HOIL-1 helix-RBR (C460A)/UbcH7(C86K)-Ub/M1 di-Ub | RNF216 RBR-helix (C688A, pSer719)/UbcH7(C86K)-Ub/mono-Ub |
|---|---|---|
Data collection | ||
Space group | P21 | C2 |
Cell dimensions | ||
   a, b, c (Å) | 83.95, 95.48, 115.00 | 152.55, 70.36, 65.15 |
   α, β, γ (°) | 90.00, 110.58, 90.00 | 90.00, 108.70, 90.00 |
Resolution (Å) | 47.74–3.08 (3.25–3.08)a | 47.64–3.03 (3.21–3.03) |
Rsym or Rmerge | 0.230 (2.624) | 0.111 (0.979) |
I/σI | 4.3 (0.9) | 5.6 (0.9) |
Completeness (%) | 99.5 (96.9) | 98.4 (95.9) |
Redundancy | 3.5 (3.6) | 3.4 (3.5) |
Refinement | ||
Resolution (Å) | 47.74–3.08 | 41.37–3.03 |
No. reflections | 31,333 | 12,667 |
Rwork/Rfree | 0.2316/0.2664 | 0.3062/0.3389 |
No. atoms | ||
   Protein | 8791 | 4401 |
   Ligand/ion | 14 | 22 |
   Water | 8 | 0 |
B-factors | ||
   Protein | 110.9 | 92.5 |
   Ligand/ion | 92.9 | 86.7 |
   Water | 59.9 | – |
R.m.s. deviations | ||
   Bond lengths (Å) | 0.002 | 0.002 |
   Bond angles (°) | 0.463 | 0.486 |
