Fig. 5: Intracellular fate of the protein corona and PS NPs illustrated by CLEM and EM micrographs.
From: Endosomal sorting results in a selective separation of the protein corona from nanoparticles
a Scheme of intracellular separation of the protein corona and PS NPs and exocytosis of NPs. Scheme was created with Biorender.com. b, b′ Protein corona-coated PS NPs in an endosome after internalization. Intracellular space is indicated with a star symbol. c, c′ Separation of the protein corona and PS NPs. Separation event is highlighted with a dotted square and an arrow. d, d′ Distribution of separated protein corona in an MVB. e, e′ Distribution of separated PS NPs in tubular recycling endosomes. f, f′ Exocytosis of separated PS NPs. Exocytosis of naked PS NPs is indicated by an arrow and intracellular space is indicated with a star symbol. Red represents the Cy5-labeled protein corona, green represents BODIPY-labeled PS NPs, yellow represents the overlay of the protein corona and PS NPs. Scale bars: 500 nm.
