Fig. 5: hab1abi1pp2ca and hab1abi1abi2 triple mutants show tolerant phenotype under low-K⁺ stress.

a Seed germination of wild type (Col-0), cbl2/3 double mutant, hab1abi1pp2ca and hab1abi1abi2 triple mutants under high- and low-K⁺ conditions. Representative images show 7-day-old seedlings after direct germination on the medium containing high-K⁺ (20 mM) condition or low-K⁺ (5 μM) condition. (b) Measurement of root length at the end of the assay as shown in (a). c Representative images of wild type (Col), cbl2/3 double mutant, hab1abi1pp2ca and hab1abi1abi2 triple mutants under high- or low-K⁺ in post-germination assays. The seedlings were grown under sufficient-K⁺ (20 mM) condition for 4 days, followed by a transfer to high-K⁺ (20 mM) or low-K⁺ (5 μM) conditions and grown for another 5 days. d Measurement of root length at the end of the assay as shown in (c). e Growth phenotype of 5-week-old wild type plants, cbl2/3 double mutant, hab1abi1pp2ca and hab1abi1abi2 triple mutants in hydroponic solutions containing high- or low-K⁺. 7-day-old seedlings germinated on MS plates were transferred to hydroponic solutions containing high-K⁺ (20 mM) or low-K⁺ (1 μM) for another 4 weeks. Photographs were taken at the end of the assay. f Measurement of the fresh weight of 5-week-old plants in hydroponic solutions as shown in e. Data in b, d, and f, are shown as mean ± SD, n = 3 (biologically independent experiments). Statistical analyses between groups were performed by one-way analysis of variance (ANOVA) followed by a Tukey’s multiple comparison test.