Table 1 Selected surface exposed residues with relatively low tolerance scores for FabZ, LpxC and MurA

FabZ

H19

0.27

H,L,M,N,Q,T

FabZ

H54

0.15

H,Q,V,Y

Catalytic residue^25,55,56,75

FabZ

F55

0.44

F,G,I,K,L,M,N,Q,V,W,Y

FabZ

P62

0.19

A,M,P,V,W

FabZ

G63

0.01

A,G

FabZ

E68

0.29

A,D,E,F,L,V

Catalytic residue^25,56,75

FabZ

A71

0.32

A,C,E,L,N,S,T,V

FabZ

Q72

0.39

A,E,G,H,L,N,Q,S

FabZ

F93

0.49

C,F,G,I,L,M,N,Q,S,V,W,Y

FabZ

G108

0.27

A,C,G,P,Q,S,T,W

FabZ

G129

0.46

A,C,D,F,G,L,N,P,S,T,V,W

LpxC

H79

0.00

H

Catalytic Zn²⁺ coordination^45,48,76

LpxC

D105

0.13

C,D,E,G

Protein stability⁴⁸

LpxC

R190

0.02

K,R

LpxC

T191

0.12

C,P,S,T

Substrate binding^45,76, stabilizing intermediates^47,50

LpxC

F192

0.12

F,I,M,W,Y

Substrate binding^45,46,50,76

LpxC

G210

0.01

A,G

Substrate binding⁴⁵

LpxC

A215

0.15

A,C,G,S,T,V

Substrate binding^45,77

LpxC

K239

0.10

M,N

Substrate binding^{46,50,76,78,79}

LpxC

D242

0.00

D

Catalytic Zn²⁺ coordination^45,76, substrate binding⁴⁶

LpxC

D246

0.00

D

Catalytic residue^45,47,48

LpxC

G264

0.04

G,S

LpxC

H265

0.05

H,Q

Catalytic residue^45,46,47,48

MurA

G114

0.00

G

MurA

C115

0.00

C

Catalytic residue^38,66,80,81

MurA

G118

0.00

G

MurA

R120

0.03

H,R

Substrate binding^37,38,39,40

MurA

S162

0.03

S,T

Substrate binding^37,38

MurA

G164

0.00

G

Substrate binding^37,38

MurA

D231

0.00

D

MurA

R331

0.02

K,R

Substrate binding^37,39,40

MurA

D369

0.02

D,E

MurA

G398

0.04

G,T