Fig. 8: Activating SREBP-1 in HK-2 cells aggravated lipid deposition. | Nature Communications

Fig. 8: Activating SREBP-1 in HK-2 cells aggravated lipid deposition.

From: Long-term statins administration exacerbates diabetic nephropathy via ectopic fat deposition in diabetic mice

Fig. 8: Activating SREBP-1 in HK-2 cells aggravated lipid deposition.The alternative text for this image may have been generated using AI.

a SREBP-1 (red) and BODIPY (green) expression was evaluated by immunofluorescence on HK-2 cells treated with negative control (Con) or insulin incubated with OA (120 μM) (Hight insulin, HI), or HI incubated with 10 μM Akt inhibitor MK2206 (HI + MK2206), 10 μM mTOR inhibitor CCI779 (HI + CCI779). FITC-labeled BODIPY, Alexa Fluor 594-labeled SREBP-1 and DAPI (nuclei, blue) were used. SREBP-1 is mainly expressed in the cytoplasmic of HK-2 cells, BODIPY in the cytoplasmic of HK-2 cells, and the specific location is indicated by the arrows. Representative image from three biologically independent samples/group were combined from three independent experiments. Original magnification ×630. Scale bar: 10 μm. b The immunoblot analysis of p-PI3K, PI3K, p-Akt (Thr308), p-Akt (Ser473), t-Akt, p-P70(S6K), P70(S6K), SREBP-1, FAS, SCD1, ACC1, HMGCR, LDLR, caspase 3, NGAL and HSP90. c Analysis of the grayscale image between them. Representative blots from three biologically independent samples/group were combined from three independent experiments. Data are expressed as means ± SEM. One-way ANOVA with Tukey post hoc test was used for the analysis of statistical significance. Significance *p < 0.05 versus CON group; **p < 0.01 versus CON group. #p < 0.05 versus HI group; ##p < 0.01 versus HI group; ###p < 0.001 versus HI group, ####p < 0.0001 versus HI group. Source data are provided as a Source Data file.

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