Fig. 1: scm⁶A-seq detects m⁶A signatures in individual germinal vesicle (GV) oocytes.

a Schematic diagram showing the scm⁶A-seq method and an overview of the samples analyzed. The carton image of oocytes and early embryos is created with BioRender. b Saturation curves for each GV oocyte data obtained by scm⁶A-seq. Each point on the curve was generated by randomly selecting a number of raw reads from each GV cell library and then using the same alignment pipeline to call covered genes. c Bar plot showing the number of detected m⁶A peaks and modified transcripts in each GV oocyte using scm⁶A-seq. d The distribution of mRNA m⁶A peaks in individual GV oocytes. e Metagene profiles depicting the normalized sequence coverage ratio of m⁶A-IP to RNA-seq data in positions surrounding the transcription start site (TSS) (left) and translation stop codon (right). The red line and light red shadow represent the mean and quantiles of 10 GV oocytes determined by scm⁶A-seq, respectively. The other three colored lines represent bulk MeRIP-seq and RNA-seq, as labeled in the panel. f Bar plot displaying the accumulative fraction of the number of the commonly m⁶A modified transcripts in 10 GV oocytes. Source data are provided as a Source data file. g The box and point plot showing the expression of common m⁶A modified transcripts. The x-axis shows the number of common m⁶A peaks in 10 GV oocytes. Source data are provided as a Source data file. h Venn diagram displaying the common m⁶A modified transcripts between single cells and bulk cells detected from MeRIP-seq data. Light green pie represents identified modified RNAs identified in 70 GV oocytes by MeRIP-seq, and the purple pies represent the common m⁶A modified RNAs form 10 individual GV oocytes as determined by scm⁶A-seq. n represents the frequency with which common m⁶A modified RNAs appeared in the analysis of the 10 individual GV oocytes.