Fig. 3: Timing of plasmid-encoded proteins production in transconjugant cells.
From: Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer
a Genetic map of the 108 kb F plasmid indicating the leading (green), Tra (red) and maintenance (blue) regions, and the positions of the studied genes (triangles). Stars represent the genetic location of the PlacIQ1sfgfp insertions. b Summary diagram of the production timing of each plasmid-encoded protein fusions in transconjugant cells with respect to the timing of ss-to-dsDNA conversion reflected by mCh-ParB focus appearance (0 min). The diagram represents data from the foldchange increase in sfGFP signal from Fig. S5. Orange/green, blue and red colours correspond to production of proteins from the leading, maintenance and transfer region, respectively. Timings of the cytoplasmic sfGFP production from the PlacIQ1 promoter inserted in the repE-sopA (repE), tnpA-ybaA (tnpA) and traM-traJ (traM) intergenic regions are represented in grey. The number (n) of individual transconjugant cells from at least three biological replicates analysed is indicated. c Jitter plots showing the intracellular green fluorescence (SNR) for each sfGFP fusions and reporters within vegetatively growing donor (left) and transconjugant cells (right) at the maximum SNR value from Fig. S5. Each dot represents data of individual cells. Means and SD are calculated from the indicated (n=) number of transconjugant cells from at least three independent biological replicates. Donors of F derivatives (see Table S1), Recipient (LY358). Source data are provided as a Source Data file.
