Fig. 2: Rhythmic DC trafficking induces lymph node homing.
From: Influence of circadian clocks on adaptive immunity and vaccination responses
a Popliteal lymph node (LN) mass 24 h following subcutaneous injection of 1 × 106 bone marrow-derived dendritic cells (BMDCs), with or without prior treatment with integrin-blocking antibodies, normalized to average ZT7 mass; n = 4–5 mice, two-way ANOVA with Sidak’s post test. b Popliteal LN cellularity 48 h following subcutaneous injection of 1 × 106 BMDCs, with and without prior treatment with integrin-blocking antibodies; n = 2–3 mice, two-way ANOVA with Sidak’s post test. c Icam1 mRNA expression in parotid LN 12 h after topical application of FITC; n = 3 mice, unpaired two-sided Student’s t test. d Time course of ICAM-1 protein levels on high endothelial venules (HEVs) of parotid LNs by quantitative imaging after topical FITC application; n = 3 mice, two-way ANOVA with Sidak’s post test. e LN cellularity 48 h after topical FITC application, with or without prior treatment with anti-ICAM1 antibody; n = 5 mice, two-way ANOVA with Fisher’s post test. f ICAM-1 protein levels on HEVs of parotid LNs 12 h after topical FITC application in WT or endothelial cell-specific Bmal1−/− mice (BMAL1ΔEC); n = 3–4 mice, two-way ANOVA with Sidak’s post test. g Chromatin immunoprecipitation (ChIP) analysis of BMAL1 binding to the Icam1 promoter region R4 in the parotid LN 6 h after topical FITC application; n = 3 mice, unpaired two-sided Student’s t test. h Time course of Tnf mRNA expression in parotid LN after topical application of FITC; n = 3 mice, two-way ANOVA with Sidak’s post test. i ICAM-1 protein levels on HEVs of parotid LNs 12 h after topical FITC application in mice pre-treated with anti-TNF antibody or isotype control; n = 5 mice, two-way ANOVA with Tukey’s post test. j Parotid LN cellularity 48 h after topical FITC application, with or without prior treatment with anti-TNF antibody; n = 8–14 mice, two-way ANOVA with Tukey’s post test. Data are plotted as mean ± standard error of mean (SEM); ns, not significant. Source data are provided as a Source data file and detailed sample sizes are available in “Methods”.
