Fig. 6: WERDS signaling complex controls apical constriction.

a Ectopic apical constriction analysis with WERDS components. Representative images of ectopic apical constriction induced by exogenously expressed Shroom3 (200 pg). The red arrowheads indicate ectopic apical constriction in the ectoderm. Data means SEM. Numbers of embryos/experiments: 82/3, 89/3, 80/3, 77/3, 85/3, 102/3, 97/3, 91/3, 103/3, and 82/3 from left to right for each column. One-way ANOVAs followed by Dunnett’s multiple comparison tests were used. n.s., not significant, ****p < 0.0001. Scale bar, 500 μm. See also Supplementary Fig. 6c. b Ectopic apical constriction analysis with the WERDS complex and ephrinB2-Δ4. Data means SEM. Numbers of embryos/experiments: 66/3, 68/3, 69/3, and 75/3 from left to right for each column. One-way ANOVAs followed by Dunnett’s multiple comparison tests were used. n.s., not significant, ****p < 0.0001. See also Supplementary Fig. 6d. c Ectopic apical constriction analysis with the WERDS complex and Ror2-ΔPR. Data means SEM. Numbers of embryos/experiments: 73/3, 70/3, 79/3, and 79/3 from left to right for each column. One-way ANOVAs followed by Dunnett’s multiple comparison tests were used. n.s., not significant, ****p < 0.0001. See also Supplementary Fig. 6f. d Ectopic apical constriction analysis with the WERDS complex and Wnt5a. Data means SEM. Numbers of embryos/experiments: 75/3, 77/3, 78/3, and 78/3 from left to right for each column. One-way ANOVAs followed by Dunnett’s multiple comparison tests were used. n.s., not significant, ****p < 0.0001. See also Supplementary Fig. 6g. e F-actin (Phalloidin) and phospho-MLC levels in ectodermal explants. Data means SEM. The number of ectodermal explants/experiments: 9/3 for each column. One-way ANOVAs followed by Dunnett’s multiple comparison tests were used. n.s., not significant, ****p < 0.0001. Scale bar, 50 μm. f A model for WERDS signal complex.