Fig. 1: circGlis3 is upregulated in and is associated with obesity.
From: Circular RNA circGlis3 protects against islet β-cell dysfunction and apoptosis in obesity
a RT-PCR analysis of circGlis3 expressive abundance. Compared to other tissues, circGlis3 is enriched in pancreas (n = 6 biological animals). b Western Blotting showing GLIS3 protein level in pancreas of the normal diet (NCD)- and high fat diet (HFD)-fed mice. c RT-PCR analysis of circGlis3 and Glis3 mRNA in pancreas of NCD- and HFD-fed mice (n = 6 biological animals). d RT-PCR analysis of circGlis3 expression in the islets of Lepob/ob mice aged from 4 to 12 weeks and HFD-fed mice from 0 to 20 weeks (e) and Leprdb/db mice aged from 4 to 12 weeks (f). (n = 6 biological subjects). g circGlis3 expression level in mouse islets and exocrine glands (n = 6 biological subjects). h. circGlis3 expression level in the sera of normal and patients with impaired glucose tolerance (IGT) (Normal individual n = 18, patients with IGT n = 21). i circGlis3 expression level in human islets exposed to different concentrations of glucose for 48 h (n = 5-6 biological subjects). a–i For bar and line graphs, data represents mean ± SEM. a, b, h Unpaired two-tailed Student’s t-test. c–f Two-way ANOVA with Bonferroni’s post-test. g, i One-way ANOVA with Tukey’s post-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source data file.
