Fig. 1: Editing capacity of ecTadA(VN)-derived ABEs.
From: TadA orthologs enable both cytosine and adenine editing of base editors
a, b Illustration of ecTadA(VN) and –derived ABE structures generated via internal insertion strategy. c, d The editing frequencies are shown in heatmap format, with adenine editing efficiencies (A-to-G editing) shown in blue and cytosine editing efficiencies (C-to-T editing) in pink gradient color. Engineered ABEs were generated in combination with internal insertion with ecTadA(VN) (c) or NLS-ecTadA(VN)-linker (NL-ecTadA(VN)) (d). Data shown here represent means of results from n = 3 biologically independent experiments. NC negative control. VN, amino acid substitutions of A106V&D108N. Base editors generated by different fusion strategies were defined as X-deaminase, with X representing fusion sites, such as N for N-terminal and 535 for internal sites after the 535th amino acid. Source data are provided as a Source Data file.
