Fig. 2: Engineering of TadA orthologs for functional ABEs, CBEs, and ACBEs.
From: TadA orthologs enable both cytosine and adenine editing of base editors
a Phylogenetic analysis of TadA orthologs with a maximum-likelihood tree and six TadA groups were defined. The evolutionary distance scale of 0.5 was shown. b Editing signatures of selected TadA ortholog-derived base editors at sgRNA-1, sgRNA-18, sgRNA-PT14, sgRNA-HPE6, and sgRNA-S5 were shown in heatmap format, with adenine editing efficiencies (A-to-G editing) shown in blue and cytosine editing efficiencies (C-to-T editing) in pink gradient color. Data shown here represent means of results from n = 2 biologically independent experiments. c Phylogenetic tree showing the relationship between 25 functional TadA orthologs. Protein lengths and substrate specificities of derived base editors were presented simultaneously. NC negative control. VN, amino acid substitutions corresponding to A106V&D108N of ecTadA. Source data are provided as a Source Data file.
