Fig. 2: Identification of 24 functional-anatomic cell groupings that preferentially interact with CSF. | Nature Communications

Fig. 2: Identification of 24 functional-anatomic cell groupings that preferentially interact with CSF.

From: Gold nanoparticle-enhanced X-ray microtomography of the rodent reveals region-specific cerebrospinal fluid circulation in the brain

Fig. 2: Identification of 24 functional-anatomic cell groupings that preferentially interact with CSF.

a Schematic of functional-anatomic regions in which 1.9 nm gold nanoparticles (AuNPs) interact with cell populations 10 min post-intraventricular injection in P7 rodents. 24 areas identified in all rodents (brown) include the nucleus accumbens (Acb), layers III and V of the cortex (III, V), dorsal medial hypothalamus (DMH), habenula (H), hippocampus (hippo), globus pallidus internus (GPi), amygdala (amyg), interpeduncular nucleus (IP), superior colliculus (SC), oculomotor nucleus (3), lateral lemniscus (LL), motor trigeminal nucleus (Mo5), periolivary region (POR), laterodorsal tegmentum (LDT), locus coeruleus (LC), ventral cochlear nucleus (VCO), facial nucleus (7, 7n), dorsal nucleus of the vagus (DMX), hypoglossal nucleus (12n), spinal trigeminal nucleus (Sp5), inferior olive (IO), deep cerebellar nuclei (DCN), nucleus ambiguus (AM), and lamina IX (IX). 1.9 nm AuNPs were also identified in the nucleus of Darkschewitsch (Dk), red nucleus (RN), trochlear nucleus (4), and nucleus of trapezoid body (NTB) in one rodent (light brown), and the nucleus sagulum (SAG), external cuneate nucleus (CU), inferior colliculus (IC), pontine reticular nucleus, and parabigeminal nucleus (PBG) in two (light brown). b Schematic showing cranial nerve nuclei with 1.9 nm AuNP uptake (brown) are primarily motor. c 1.9 nm AuNP mean intensity in the 24 regions. Data are mean ± S.D., n = 3 rodents. d X-ray microtomography (XRM) images showing 1.9 nm AuNP distribution. scalebars = 1 mm. e Coronal XRM (left) and histology (right) showing 1.9 nm AuNP distribution in the 12n (orange box). XRM scalebar = 1 mm, histology scalebar = 25 µm. f–k Histology showing similar 1.9 nm AuNP distribution patterns in the cortex (f), hippocampus (g), hypothalamus (h), Mo5 (i), 7n (j), and 12n (k) after intraventricular (ICV) and intra cisterna magna (ICM) injection in P7 rats. scalebars = 50 µm. l, m Confocal microscopy and XRM showing similar distribution patterns in the cortex, LC, Mo5, and 7n following ICM injection of Red-Dextran/tetramethylrhodamine (RD/TMR) and ICV 1.9 nm AuNP. scalebars = 1 mm. n–p Fluorescence microscopy and XRM showing similar distribution patterns in the fasciculus retroflexus (fr), RN, pyramidal tract (py), IP, oculomotor complex, 4, LDT, Mo5, 7, VCO, Sp5, DCN, and CU between a P8 mouse injected with ICM CellTracker (CT) and a P7 rat injected with ICV 1.9 nm AuNPs. scalebars = 1 mm. Source data are provided in Supplementary Information. Figure 2a, b were created with BioRender.com.

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