Fig. 6: Increased DRG synchrony elicits S1 plasticity and pain-like behavior in naive mice.

a Schematic of peripheral BzATP or ATP application. b Representative fluorescence traces of DRG neurons with application of BzATP or ATP. c Integrated Ca²⁺ activity of the DRG neurons induced by local application of 500 μM BzATP was comparable to that induced by peripheral application of 10 mM ATP. d Cross-correlograms of the same 10 neurons after BzATP (top panel) and ATP application (bottom panel). e Correlation coefficient of DRG neurons before and after ATP or BzATP application in naive mice. f Schematic of experimental design. In vivo Ca²⁺ imaging of L2/3 pyramidal neurons and ECoG recording were performed in S1 of naive mice treated with saline, BzATP, or ATP for 3 days. g, h Integrated Ca²⁺ activity (g) and correlation coefficient (h) of L2/3 pyramidal neurons in naive mice after repeated saline, BzATP, or ATP treatment. i, j ECoG power spectra (i) and the theta band power (j) in naive mice with saline, BzATP or ATP treatment for 3 days. k Schematic of in vivo apical spine imaging in S1 of naive mice treated with saline, BzATP or ATP for 3 days. l, m Percentages of apical dendritic spines of L5 pyramidal neurons formed (l) and eliminated (m) over 1 day, 2 days, or 3 days in the S1 of naive mice with repeated saline or BzATP or ATP treatment. n Measurement of PWT in naive mice with repeated treatment of saline, BzATP or ATP. o Preference scores (difference in time spent between lidocaine- and saline-paired compartment) indicative of spontaneous pain were examined in naive mice after repeated treatment of saline, BzATP or ATP for 3 days. Data are expressed as mean  ±  SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. See Table S1 for statistical details. Source data are provided as a Source Data file.