Fig. 2: HMG20A binds NuRD complex components and DNA. | Nature Communications

Fig. 2: HMG20A binds NuRD complex components and DNA.

From: The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs

Fig. 2

A Anti-GFP immunoprecipitations of HEK293 cell extracts co-transfected with GFP-HMG20A and FLAG-HDAC1, -MTA1, -MTA2 and -RBBP4. Proteins were detected with anti-FLAG or anti-GFP antibodies. B Top: Anti-GFP immunoprecipitations of HEK293 cell extracts co-transfected with GFP-HMG20A and FLAG-CHD4 (CHD4), -CHD4-N-terminus (CHD4-N), -CHD4-middle domain (CHD4-M), -CHD4-C-terminus (CHD4-C) and HA-GATAD2A, -MBD2 and -MBD3. Proteins were detected with anti-FLAG and anti-HA or anti-GFP antibodies. Bottom: schematic depiction of CHD4 deletion constructs. C Top: schematic depiction of HMG20A deletion constructs. Bottom: anti-GFP IPs of HEK293 cell extracts co-transfected with GFP-HMG20A and its deletions (HMG, CC) and of NuRD members (FLAG-MTA1, HDAC1-FLAG, FLAG-RBBP4). Proteins were detected with anti-FLAG or anti-GFP antibodies. D EMSA of increasing amounts of extracts from Sf9 cells expressing FLAG-HMG20A and its deletions (see C, top) using Cy5-labelled DNA. Experiments in A-E were repeated independently at least two times with consistency. Source data for these figures are provided as a Source Data file.

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