Fig. 1: A time-resolved map of STING trafficking.
From: ESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling
a Schematics of STING-TurboID fusion. STING was fused to TurboID at the CTT. Addition of biotin allows labeling of neighboring proteins in a 10 nm radius. Labeled proteins can be then enriched after cell lysis with streptavidin pulldown. b Immunoblot of Streptavidin-HRP (Strept.), STING and TBK1 in input and post streptavidin pull-down (PD: strept.) after 2 µg/ml cGAMP stimulation (in perm buffer) for the indicated times in 293T STING-TurboID. One representative experiment of n = 3 experiments. Marker unit is KDa. c Scheme of the time-course used for STING-TurboID proteomics. Reporter TMT labeling ions used for each condition are indicated. d STRING generated network of filtered STING interactors after statistical analysis. Colors represent annotation of cellular compartments. Proteins were filtered on adj. pvalue < 0.07 to include TBK1 (adj. pvalue = 0.0611). e Relative enrichment of proteins as in d) at the different time points. f Heat-map of the filtered proteins with enrichment at the different timepoints. n = 2 per time-point.
