Fig. 4: ESCRT links STING degradation and autophagy resolution at the endosome.
From: ESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling
Immunofluorescence of a CD63 (cyan), HGS (magenta) and STING (yellow), b p62 (cyan), HGS (magenta) and STING (yellow), c LC3B (cyan), HGS (magenta) and STING (yellow), d p62 (cyan), CD63 (magenta) and STING (yellow) in 293T stably expressing STING-HA non stimulated (left panels) or after stimulation with 2 µg/ml cGAMP (in perm buffer) for 2 h (right panels). Dashed box represents the cropped region shown in the enlarged panels. Green dashed line represents the line used to plot normalized pixel intensity for each protein. Brackets in the profile in panel a represent proximity of STING/HGS peaks to CD63 peaks. Panels b, c and d show also an enlargement of the structures indicated by the white arrow. One representative field of n ≥ 3 fields in n = 3 independent experiments. Scale bar is 10 µm for wide-field and 3 µm for enlargement. Corresponding single color images are in Fig. S5.
