Fig. 4: Incorporation of myoglobin within SAPs improves graft survival and graft innervation. | Nature Communications

Fig. 4: Incorporation of myoglobin within SAPs improves graft survival and graft innervation.

From: Hydrogel oxygen reservoirs increase functional integration of neural stem cell grafts by meeting metabolic demands

Fig. 4

a Volume of graft core in Cell (n = 5), Gel (n = 8), Horse (n = 9), Whale (n = 4), Low affinity whale (n = 4) and High affinity whale (n = 6) groups. Boxes show medians with interquartile ranges, whiskers: min to max show all points. (Cell vs Horse *p = 0.0195, Gel vs Horse *p = 0.0488, Cell vs High affinity whale *p = 0.0086, Gel vs High affinity whale *p = 0.0246, Horse vs Low affinity whale *p = 0.001, Horse vs Whale *p = 0.0084, High affinity whale vs Low affinity whale *p = 0.025, High affinity whale vs Whale *p = 0.0063) b Volume of innervation in Cell (n = 5), Gel (n = 8), Horse (n = 9), Whale (n = 4), Low affinity whale (n = 4) and High affinity whale (n = 6) groups. Boxes show medians with interquartile ranges, whiskers: min to max show all points. (Cell vs High affinity whale *p = 0.045, Gel vs High affinity whale *p = 0.0069, Horse vs High affinity whale *p = 0.0437, Low affinity whale vs High affinity whale *p < 0.0001, Whale vs High affinity whale *p = 0.0009, Gel vs Horse *p = 0.048, Low affinity whale vs Horse *p = 0.0029, Whale vs Horse *p = 0.0192) c Representative photomicrographs providing a coronal view of GFP + grafts in cell only, hydrogel and Mb:hydrogel groups, respectively. Graft core is delineated in green. Scale bar represents 500 μm. The representative images in 4c show a summary of at least three independent experiments. Data are presented as mean ± standard error of the mean (SEM) (*, p < 0.05). One-way ANOVA with Tukey’s post hoc test for multiple comparisons and Unpaired two-sided t-test were used for statistical analysis. Source data are provided as a Source Data file.

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