Fig. 4: Simultaneous in situ detection of DNA, RNA, and proteins by π-FISH rainbow.
From: Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules
a A putative translocation site between chromosomes 9 and 11 in THP-1 cells according to the DLO Hi-C data. b, c Detection of translocation sites in chromosomes 9 (green) and 11 (red) by π-FISH rainbow in THP-1 cells (d) and control HEK293T cells (e) confirmed the translocation in THP-1 cells. Scale bars, 2.5 μm. d Schematic for detecting seven loci on the long arm of chromosome 22 by π-FISH rainbow based on single and merged signals from three fluorescent channels: Ch 1, channel 1; Ch 2, channel 2; Ch 3, channel 3. e The hybridization signals for seven loci (rectangular box i and ii) on the long arm of chromosome 22 were decoded and reconstructed in the 3D image. Scale bar, 3 μm. f Homologous chromosome structure of the long arm of chromosome 22 based on hybridization signals. g Simultaneous detection of lncRNA NEAT1 and its target MAPK15 genomic locus in HeLa cells was imaged by confocal Z-axis scanning. NEAT1 and MAPK15 signal probes were labeled with Alexa Fluor 647 (magenta) and Alexa Fluor 488 (green), respectively. Scale bar, 2.5 μm. h Simultaneous detection of Pol II protein (red), NEAT1 mRNA (magenta), and its target genomic locus (MAPK15, green) using π-FISH rainbow in HeLa cells followed by confocal Z-axis scanning. Scale bar, 2.5 μm.
