Fig. 1: A constrictive bottleneck impedes C. rodentium enteric colonization.
From: Quantitative dose-response analysis untangles host bottlenecks to enteric infection
a Experimental strategy. To quantify the contraction (bottleneck) of the C. rodentium population during colonization a barcoded library of bacteria was orally administered to mice. The number of unique cells from the inoculum (founders) that expand to produce the observable population (burden) was determined from the frequency and diversity of barcodes. b 5 cohoused, adult, female, C57BL/6 J mice were administered 4 × 108 CFU STAMP-CR253 by oral gavage and the C. rodentium population was monitored in the feces for 5 days post inoculation (p. i.). Dose and burden were enumerated by serial dilution and plating; founders were determined by STAMP. Lines and error are geometric means and standard deviation. Bacteria not detected (ND) counted as 0.5, CFU colony forming units, g grams. Validation of STAMP libraries in Supplemental Fig. 1. Source data are provided as a Source Data file.
