Fig. 2: Localization and high-resolution reconstruction of PBS-PSII complex.
From: Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA
a 3D PBS model (EMD-9976) used in the projection matching, and the molecular weight is about 14.7 MD. b Locations on the image from projection matching at threshold 8. c Isosurface of the 3.4 Å-resolution PBS map colored by local resolution, showing a high resolution near the core and a lower resolution outside may due to the flexibility. d The FSC curve of PBS structure. e High-resolution features of PBS map fitted by pdb 6kjx, such as amino-acid side chains (left) and β-strands (right), are clearly identified for this resolution range. f Overall structure of PBS-PSII complex, with 3 PSII dimers (PSII 1 in blue, PSII 2 in green and PSII 3 in orange) connected to the PBS. g FSC curves of three PSII dimers, indicating the resolutions of 3.6 Å (blue, PSII 1), 3.7 Å (green, PSII 2) and 4.4 Å (orange, PSII 3) at FSC 0.143. h Extra densities in PBS map (colored in light green). Source data are provided as a Source Data file.
