Fig. 2: Erasing H3K79me2 prior to ionizing radiation exposure reduces cGAS recruitment to micronuclei.

a Percent cGAS+ MN by immunofluorescence (IF), 72 h post-exposure to 10 Gy IR in MCF10A. Statistical comparisons by Student’s t-test using DMSO treatment as the reference group. Bonferroni-adjusted p values are displayed for each comparison, with statistically significant comparisons indicated with asterisks. See Supplementary Table S1 for a list of protein targets corresponding to each inhibitor in this screen. b Percent cGAS+ MN by immunofluorescence (IF), 72 h post-exposure to 10 Gy IR in MCF10A. Cells were pre-treated as indicated. c FLAG-cGAS co-immunoprecipitated (IP) from each of the indicated conditions, 72 h following 10 Gy IR. Blot representative of 3 experiments. d, e ISG54 and ISG15 expression by RT-qPCR, 72 h post-10 Gy IR in MCF10A. Indicated cells were pre-treated for 7 days with DOT1Li; 24 h with herring testis (HT)-DNA; or 24 h with cGAMP prior to IR exposure. All statistical comparisons performed using a two-sided Student’s t-test. ns: p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. All individual data points presented for immunofluorescent scoring of MN represent the mean percentage of MN that were positive for the indicated marker, from each biological replicate out of 50 total MN per replicate. All error bars represent standard error of the mean, for three independent biological replicates. Source data are provided as a Source Data file.