Table 2 Scoring of RNAscope ISH and IHC signal in brain slices
Score | Description |
|---|---|
Negative | No specific staining detectable |
Uncertain | Very few signals, not clearly associated with cellular structures |
1 | Signals in the cytoplasm or processes of singular neurons or glial cells |
2 | Signals in an average of 5–10 cells per field (in 40-fold magnification in a 0.237 mm2 field area) |
3 | Signals in an average of more than 10 cells (up to ~100) per field (in 40-fold magnification in a 0.237 mm2 field area) |
