Fig. 10: The reverse transcriptase inhibitor Lamivudine ameliorates fibroinflammation.

a Experimental scheme. b Relative DNA content of Etnii in kidneys of mice underwent sham or UUO surgery and injected with vehicle or 3TC (CTL SHAM, blue; CTL UUO, red; 3TC SHAM, black; 3TC UUO, green; n = 5 in each). c Representative images of Western blots of RIG-I, MDA5, cGAS, pSTING, STING, pTBK1, TBK1, pP65, P65, pIRF3, IRF3, FN, and SMA protein levels in kidneys of sham and UUO mice injected with vehicle or 3TC. GAPDH was used as a loading control. d Relative mRNA levels of ISGs (Mx1, Isg15, Ifitm1, Bst2, and Oas1) in kidneys of SHAM and UUO mice injected with vehicle or 3TC (n = 5 in each). e Representative hematoxylin and eosin (H&E) staining of kidneys from sham and UUO mice injected with vehicle or 3TC. Scale bar, 10 μm. f Representative images of Sirius red staining of kidneys from sham and UUO mice injected with vehicle or 3TC. Scale bar, 10 μm. g The degree of fibrosis score was quantified by Sirius red staining of kidneys from sham and UUO mice injected with vehicle or 3TC (n = 5 in each). The data are represented as mean ± s.e.m. and all data were analyzed using a one-way ANOVA followed by Tukey post hoc test for multigroup comparison (b, d, g). Data were representative of two independent experiments (e, f). The blots shown are representative of n = 2 biological replicates. Source data are provided as a Source Data file.