Fig. 4: Increased dsRNA content in UUO mice kidneys elicits innate immune response via RIG-I.

a dsRNA content in SHAM and UUO mice kidneys. Data were representative of two independent experiments (IgG, Black; dsRNA SHAM, blue; dsRNA UUO, red). b Relative mRNA levels of cytoplasmic nucleic acid sensors (Rigi, Sting, Cgas, and Mda5) in WT and STING KO PTECs untreated or transfected with immunoprecipitated RNA using IgG or dsRNA antibody (n = 4 in each). c Relative mRNA levels of ISGs (Mx1, Isg15, Ifitm1, Oas1, Ch25h, and Bst2) in WT and STING KO PTECs transfected with immunoprecipitated RNA using IgG or dsRNA antibody (n = 4 in each). d Ifnb, Ifne, and Ifnk RNA levels in WT (untreated, white; IgG, gray; dsRNA, black) and STING KO (IgG, pink; dsRNA, red) PTECs transfected with immunoprecipitated RNA using IgG or dsRNA antibody (n = 4 in each). e CO-IP showing the interaction between dsRNA and RIG-I. Kidney lysates of mice with UUO injury were used for immunoprecipitation using IgG control and dsRNA antibody followed by western blot using RIG-I antibody. f Reverse CO-IP validating the interaction between RIG-I and ERVs. Kidney lysates from mice with UUO injury were used for immunoprecipitation using IgG control (gray) and RIG-I antibody (pink) followed by RNA isolation and qRT-PCR. The graph shows enrichment in Class I ERVs (xMlv, pMlv, mpMlv, and Mmtv), Class II (Musd and Iaps), and Class III (Malr) bound with RIG-I protein. Data were representative of two independent experiments. The data were represented as mean ± s.e.m. and all data were analyzed using a one-way ANOVA followed by Tukey post hoc test for multigroup comparison (b–e). Source data are provided as a Source Data file.