Fig. 8: RIG KO STING KO (Double KO) mice were protected from FA-induced injury.

a Relative mRNA levels of Rigi and Sting in kidneys of WT (vehicle, blue; FA, red) and RIG KO STING KO mice (vehicle, black; FA, green) injected with FA (n = 5 in each). b Relative RNA levels of other cytosolic RNA sensors (Cgas, and Mda5) in kidneys of WT and RIG KO STING KO mice injected with FA (n = 5 in each). c Relative mRNA level of ISGs (Mx1, Isg15, Oas1, Ch25h, and Bst2) in kidneys of WT and RIG KO STING KO mice injected with FA (n = 5 in each). d Serum BUN levels of WT and RIG KO STING KO mice injected with FA (n = 5 in each). e Representative images of H&E staining of kidney sections from WT and RIG KO STING KO mice injected with FA. Scale bar: 10 µm. f Relative mRNA level of fibrosis markers (Tgfb1, Col1, Col3, Vim, and Fn) in kidneys of WT and RIG KO STING KO mice injected with FA (n = 5 in each). g Representative images of Sirius red staining of kidney sections from WT and RIG KO STING KO mice injected with FA. Scale bar: 10 µm. h Quantification of tubulointerstitial fibrosis by Sirius red staining in WT and RIG KO STING KO mice injected with FA (n = 5 in each). The data are represented as mean ± s.e.m. and all data were analyzed using a one-way ANOVA followed by Tukey post hoc test for multigroup comparison (a–d, f, h). Data were representative of two independent experiments (e, g). Source data are provided as a Source Data file.