Fig. 3: Phylogenetic and genetic repeats of intense nucleotide skew (GRINS) analysis of AZL PKS. | Nature Communications

Fig. 3: Phylogenetic and genetic repeats of intense nucleotide skew (GRINS) analysis of AZL PKS.

From: Insights into azalomycin F assembly-line contribute to evolution-guided polyketide synthase engineering and identification of intermodular recognition

Fig. 3

a Phylogenetic analysis of AZL PKS modules. Each bar represents a domain in PKS, with ATa the domain that loads a malonyl-CoA to build an acetate unit and ATp the domain that loads a methylmalonyl-CoA to build a propionate unit. The numbers in the dotted frame show identities and positives of the ATp-DH-ER tridomain between modules 3 and 6. b GRINS in AZL PKS. Duplicated regions (>80% DNA sequence identity to another region within the same PKS) containing the intense GC and AT skews are annotated as GRINS. Although sequences in AZL PKS assembly-line are highly similar (the black curve), GRINS only are present in several regions (the blue shade). The highly identical GRINS in module 3 and module 6 are outlined in yellow.

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