Fig. 4: Mutational analyses of the binding interface between CagZ and Cagβ-AAD1 using ITC. | Nature Communications

Fig. 4: Mutational analyses of the binding interface between CagZ and Cagβ-AAD1 using ITC.

From: Mechanism of regulation of the Helicobacter pylori Cagβ ATPase by CagZ

Fig. 4: Mutational analyses of the binding interface between CagZ and Cagβ-AAD1 using ITC.The alt text for this image may have been generated using AI.

Different colors indicating the different levels of affinity compared with the wild type (Black, nearly the same affinity with the wild type; violet, 2–10 fold weaker; blue, 50-1000 fold reduced; green, binding undetectable). a high affinity binding between wild type CagZ and Cagβ-AAD1. b The F149A mutant of CagZ and the F358A mutant of Cagβ-AAD1 have significantly reduced binding affinity. c The effects of left side interface mutants on the binding affinity. d Binding affinities of various C-terminal tail peptides of CagZ for Cagβ-AAD1.

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