Fig. 1: Tomato chromatin architecture displays a strong compartmentalization and is organized around transcription factories. | Nature Communications

Fig. 1: Tomato chromatin architecture displays a strong compartmentalization and is organized around transcription factories.

From: HSFA1a modulates plant heat stress responses and alters the 3D chromatin organization of enhancer-promoter interactions

Fig. 1

a Immunofluorescence detection of H3K9ac (blue) and H3K27me1 (orange) histone modifications and DAPI staining (grey) in an isolated tomato nucleus. 3 times each experiment was repeated independently with similar results. b Chromosome 10 Hi-C interaction matrix at increasing levels of resolution. H3K9ac ChIP-seq signal (blue) and H3K27me1 signal (orange) were aligned with the maps to highlight the correlation with compartmentalization in tomato. c Pearson correlations of distance-normalized Hi-C interaction frequency maps of chromosome 10. The ChIP-seq signal for H3K9ac (blue) and H3K27me1 (orange) as well as the -PC1 component from principal component analysis (black) were aligned to the map. d Previous interaction matrix with reorganized bins according to their PC1 value. e Chromosome 1 interaction matrix obtained by calculating the difference between the RNAPII HiChIP and the Hi-C signal. Red dots are interactions enriched in RNAPII HiChIP compared to Hi-C, and blue dots are interactions enriched in Hi-C compared to RNAPII HiChIP.

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