Fig. 3: Distal and proximal REs displayed different chromatin signatures. | Nature Communications

Fig. 3: Distal and proximal REs displayed different chromatin signatures.

From: HSFA1a modulates plant heat stress responses and alters the 3D chromatin organization of enhancer-promoter interactions

Fig. 3

a Heatmaps of ATAC-seq and ChIP-seq (H3K4me3, H3K9ac, H3K18ac and H3K27ac) profiles over proximal (grey) and distal (purple) regions at 0, 1 and 6 h of heat stress. b Example of a distal regulatory element (RE) and of the histone modifications surrounding it after 0, 1 and 6 h of heat stress. c Heatmaps of ATAC-seq signal and RNAPII occupancy over distal REs at 0, 1 and 6 h after heat stress. d K-means clustering of distal differentially accessible chromatin regions and the corresponding top enriched motif identified by HOMER (v4.11) for each cluster. Binomial distribution test was used in detected ATACseq peaks. e Example of two types of transient chromatin loop formation: a promoter-centric hub and a RE-centric hub. Significant interactions for C-Hi-C and HiChIP-RNPII data were detected with HOMER (v4.11) using cumulative binomial distribution with p value 0.05.

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