Fig. 3: Molecular model of ribosome-bound RQT.
From: Structural basis for clearing of ribosome collisions by the RQT complex
a Domain arrangement of Slh1 and closest homologs Brr2 and Ski2. Slh1 was completely modeled except for the N-terminus (gray). RecA1, RecA2 = RecA-like ATPase domains, WH = winged helix domain, Sec63 = Sec63-like domain (subdivided into Ratchet-, helix-hairpin-helix (HhH) and fibronectin type III (FN3) domains). b Molecular model of RQT bound to the 80S ribosome in C1 state. On the right side RQT and the 80S ribosome were converted to low-pass filtered density for clarity. RQT-ribosome interaction regions are highlighted in violet. The mRNA entry site is marked as a blue circle. A thumbnail indicates the overall orientation NTC, CTC = N-terminal and C-terminal cassette. c View focusing on the interaction of Slh1-NTC with the 40S. d Zoomed-in view showing the interaction of the N-terminal Sec63 homology region of Slh1 with 40S uS3. e View focusing on the Cue3 N-terminal (helical) domain interacting with Slh1. f View focusing on Rqt4 interacting with Slh1. In (e) and (f) schematic representations of Cue3 and Rqt4 are shown. Modeled residues are shown in strong, unmodeled regions in weak color; dashed orange and pale, yellow lines outline NTC and CTC of Slh1.
