Fig. 5: Biofilm properties of A. baumannii AB6870155 and K. pneumoniae KP6870155 co-cultures. | Nature Communications

Fig. 5: Biofilm properties of A. baumannii AB6870155 and K. pneumoniae KP6870155 co-cultures.

From: Cross-protection and cross-feeding between Klebsiella pneumoniae and Acinetobacter baumannii promotes their co-existence

Fig. 5

a RNA-seq expression changes (log2FC) of AB6870155 and KP6870155 co-cultured biofilms relative to their respective mono-cultured biofilms. b Growth (bars) and biofilm formation (points) (measured as OD550 after staining biofilms with crystal violet) of various ratios of AB6870155: KP6870155 grown planktonically; biofilm production detected by crystal violet assay (n = 8) and data are presented as mean values + /− SEM. c CLSM images of mono-culture and dual-species biofilms grown in flow-cell chambers for 3 days and stained using BacLight Live/Dead stain (ThermoFisher); green—live cells, red—dead cells. d SEM images of mono-culture and dual-species biofilms grown in microculture plates for 21 h (n = 3 independent bacterial cultures). e Physical appearance of single species or dual-species biofilms grown on coverslips for 21 h. f Mean cell length of pure versus co-culture biofilms grown on coverslips and imaged with SEM (AB + KP = AB6870155 + KP6870155 co-cultures); MicrobeJ software used to calculate cell lengths in SEM images. P-values were calculated using a two-sided Mann–Whitney test where significance values represent: **p ≤ 0.02, non-significant p-value = ns where p > 0.05. Boxes are bound by the first and third quartile with a horizontal line at the median and whiskers represent 1.5x the interquartile range. Dots represent individual cells (n = 172 AB + KP, n = 225 AB6870155, n = 21 KP6870155). Source data are provided as a Source Data file.

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