Fig. 2: Growth adaption of E. coli to AA downshift during elevated (p)ppGpp level.
From: Stringent response ensures the timely adaptation of bacterial growth to nutrient downshift
E. coli was transformed with pLAS13 plasmids, in which a constitutively active relA* (encoding the N-terminal 1-455 aa of native relA) was driven by the IPTG-inducible Ptac promoter. A Effect of relA* overexpression (OE) on the growth rate of E. coli in glucose cAA medium. IPTG was added at a concentration of 30 µM. Error bars are the standard deviations of several biological replicates (n = 8 and 5 for wild type and relA* OE, respectively). B Effect of relA* overexpression on the cellular ppGpp pools of E. coli in glucose cAA medium. IPTG was added at a concentration of 30 µM. Error bars are the standard deviations of several biological replicates (n = 6 and 3 for wild type and relA* OE, respectively). C Growth of E. coli (pLAS13-relA*) strain during transition from glu+cAA medium to glucose minimal medium. IPTG was added at a concentration of 30 µM. D Lag time of E. coli (pLAS13-relA*) strain during AA downshift vs. IPTG concentration. Error bars are the standard deviations of several biological replicates (n = 3). Source data are provided as a Source Data file.
