Fig. 5: Growth curve and lag time of E. coli and V. natriegens during carbon downshift.
From: Stringent response ensures the timely adaptation of bacterial growth to nutrient downshift
A Growth and lag of E. coli wild type and relA-deficient strains during transition from glucose medium to lactose medium. B Growth and lag of E. coli wild type and relA-deficient strains during transition from glucose medium to glycerol medium. C Growth and lag of E. coli wild type and relA-deficient strains during transition from glucose medium to acetate medium. D Lag time of E. coli during carbon downshift using filtration method. Error bars are the standard deviations of several biological replicates (wild type strain: n = 3, 3, and 5 for lactose, glycerol, and acetate, respectively; relA-deficient strains: n = 3, 7, and 8 for lactose, glycerol, and acetate, respectively). E Lag time of E. coli during carbon diauxie, in which the secondary carbon source (lactose or glycerol) is supplemented together with glucose to the culture. Error bars are the standard deviations of several biological replicates (n = 3 for lactose; n = 4 for glycerol). F The ppGpp pools of E. coli at 0 min, 5 min, and 20 min after transition from glucose medium to acetate medium. Error bars are the standard deviations of several biological replicates (for 0 min condition, n = 6 and 4 for wild type and relA-deficient strain, respectively; n = 3 for the rest conditions). G Lag time of V. natriegens during glucose to glycerol transition. Error bars are the standard deviations of several biological replicates (n = 4). H Lag time of V. natriegens during glucose to acetate transition. Error bars are the standard deviations of several biological replicates (n = 3 and 6 for wild type and relA-deficient strain, respectively). Source data are provided as a Source Data file.
