Fig. 6: The induction kinetics of catabolic operons in E. coli during carbon downshift.
From: Stringent response ensures the timely adaptation of bacterial growth to nutrient downshift
A The central catabolic pathways of E. coli for related carbon sources. B Three catabolic operons. C, D The induction kinetics of lac mRNA in E. coli wild type strain and relA-deficient strain during glucose to lactose transition. E The induction kinetics of LacZ protein in E. coli wild type strain and relA-deficient strain during glucose to lactose transition. F, G The induction kinetics of glpFK mRNA in E. coli wild type strain and relA-deficient strain during glucose to glycerol transition. H, I The induction kinetics of glpD mRNA in E. coli wild type strain and relA-deficient strain during glucose to glycerol transition. J, K The induction kinetics of aceBA mRNA in E. coli wild type strain and relA-deficient strain during glucose to acetate transition. L, M The relative abundances (LFQ intensity) of aceB and aceA mRNA (detected by their 3’-tail region primers) of E. coli at different time points after shifting from glucose to acetate medium. Error bars are the standard deviations of three biological replicates (n = 3). N The relative abundances of AceA and AceB proteins of E. coli at different time points after shifting from glucose to acetate medium. O Effect of (p)ppGpp induction on the levels of AceA and AceB proteins. Overexpression of RelA* (Ptac-relA* induced by 30 µM IPTG) was used to increase the cellular (p)ppGpp pools of E. coli. P The effect of AceBA overexpression on the lag time of E. coli during glucose to acetate transition. Data originated from Balakrishnan et al.14; Q Growth rate of wild type strain and RelA* overexpression strain (30 µM IPTG) in glucose minimal medium. n = 9 and 2 for wild type and relA-deficient strain, respectively. R The growth and lag of RelA* overexpression strain (measured by microplate reader). S The effect of RelA* overexpression on the lag time of E. coli. Error bars are the standard deviations of at least four biological replicates (n = 5 and 4 for wild type and relA* OE, respectively). Source data are provided as a Source Data file.
