Fig. 3: Confocal images using red fluorescent silica nanoparticles. | Nature Communications

Fig. 3: Confocal images using red fluorescent silica nanoparticles.

From: Quantifying PON1 on HDL with nanoparticle-gated electrokinetic membrane sensor for accurate cardiovascular risk assessment

Fig. 3

a–g Use fluorescent Si-NP and h–n uses fluorescent Si-NP' both added directly after sample incubation. All the images of membranes had anti-ApoAI as the capture antibody except for g and n that had Anti-ApoB. The images show the state of membrane for sample containing a 1 pM, b 10 pM, c 100 pM, d 1 nM and e 10 nM of PON1-HDL and h 1 pM, i 10 pM, j 100pM, k 1 nM and l 10 nM of HDL-P. A protein cocktail containing rePON1, reApoAI and HSA (1:40:1000) that contained an equivalent protein in 10 nM PON1-HDL and total HDL respectively, i.e., f 10 nM rePON1 + 400 nM reApoAI + 10000 nM HSA and m 1 nM rePON1 + 40 nM reApoAI + 1000 nM HSA were used as controls. g and n used 10 nM of HDL as sample but with anti-ApoB, thus providing no signal. Scale bars are 100\(\mu\)m. Imaging was done once for each case.

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