Fig. 5: TN1 affects the expression of abscisic acid (ABA) biosynthetic gene TaNCED3 and ABA content in the shoot base and tiller bud. | Nature Communications

Fig. 5: TN1 affects the expression of abscisic acid (ABA) biosynthetic gene TaNCED3 and ABA content in the shoot base and tiller bud.

From: Tiller Number1 encodes an ankyrin repeat protein that controls tillering in bread wheat

Fig. 5: TN1 affects the expression of abscisic acid (ABA) biosynthetic gene TaNCED3 and ABA content in the shoot base and tiller bud.The alternative text for this image may have been generated using AI.

a, b Morphology of the shoot base (a) and tiller buds (b) used for RNA-seq and ABA/IAA contents analysis. The tiller buds are indicated with red lines. Scale bars, 1 mm in (a), 2 cm in (b). c Heatmap representation of the expression levels of DEGs in the shoot base and tiller buds shared between YZ4110 and the tn1 mutant. d RT-qPCR analysis showing the expression patterns of TaNCED3 genes in YZ4110 and the tn1 mutant. TaNCED3-5B, TraesCS5B02G029300; TaNCED3-5D, TraesCS5D02G038800. The relative expression levels were normalized to TaGAPDH. Data are means ± SD of six biological replicates. e, f Measurement of ABA and indole-3-acetic acid (IAA) contents in the shoot base and tiller buds of the tn1 mutant, respectively. Data are means ± SD of three biological replicates. The p values in d–f are indicated by two-tailed unpaired t-test. Source data are provided as a Source Data file.

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