Fig. 1: FRET-CLEM. | Nature Communications

Fig. 1: FRET-CLEM.

From: A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells

Fig. 1: FRET-CLEM.The alt text for this image may have been generated using AI.

a Correlative FLIM-FRET and PREM images of unroofed membranes of HeLa cells expressing EGFP-CLC (top) or EGFP-ShadowY-CLC (bottom). FLIM images (left; photon counts are represented by brightness and fluorescence lifetimes are represented by pseudo color), PREM images (center), and merge images (right). n = 1 cell for each condition. Scale 500 nm. b PREM images of CCSs on an unroofed membrane of a HeLa cell expressing EGFP-CLC that were classified as flat, domed, or sphere and corresponding areas from a FLIM image. n = 1 cell. Scale 200 nm. c Fluorescence lifetime decays from single CCSs indicated by arrows in panel a. d Mean fluorescence lifetimes from single CCSs on an unroofed membrane of HeLa cells expressing EGFP-CLC or EGFP-ShadowY-CLC. n = 58 CCSs from 1 cell (EGFP-CLC) and 81 CCSs from 1 cell (EGFP-ShadowY-CLC). For box plots, box is interquartile range, center line is median, center circle is mean, whiskers are minimum and maximum data points with a coefficient value of 1.5. Source data are provided as a Source Data file.

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