Fig. 2: Monocyte-like dendritic cells exhibit high diversity and a pro-angiogenic phenotype during GCPM.

a Dotplot showing antigen-presenting, pro-angiogenic, phagocytotic, pro-inflammatory, anti-inflammatory, and proliferative function scores of myeloid-derived cell clusters in G0-G3 Groups. Dot size represents percent of expressing cells in each cluster, color represents z-score of normalized mean expression level of selected gene signatures. DC dendritic cell, Macro macrophage, Mono monocyte. b Violin plot showing expression levels of selected antigen-presenting and pro-angiogenic genes in dendritic cell (DC) clusters among G0 (n = 4 samples), G1 (n = 4 samples), G2 (n = 10 samples) and G3 (n = 12 samples) Group, color-coded by cell type. Horizontal dotted line represents mean value, and colored dotted curve line indicates changes in expression level of selected genes. Box represents median ± interquartile range; whiskers represent 1.5x interquartile range; p-values are calculated by two-sided unpaired Wilcoxon test. c Developmental trajectory plot of dendritic cell (DC) clusters, color-coded by cluster (left) and pseudotime (right). Each dot represents a single cell. d Curve plots showing metabolism score changes related to glycolysis, fatty acid metabolism, and oxidative phosphorylation in dendritic cells (DCs) along pseudotime. Point colors correspond to cell type colors in c. e The cell distribution of each dendritic cell (DC) cluster along with the pseudotime (upper panel), color-coded by DC clusters. Heatmap showing dynamic expression changes of genes in DC clusters (lower panel). f Curve plots showing expression level changes of function-related genes related to antigen-presentation, pro-angiogenesis, proliferation, plasticity, and immune checkpoint along pseudotime in dendritic cells (DCs). Point colors correspond to cell type colors in c. Source data are provided as a Source Data file.