Fig. 4: SnDNA-seq identified two mutually exclusive clones bearing two different KRAS mutations in one pancreatic cancer patient.
From: Application of high-throughput single-nucleus DNA sequencing in pancreatic cancer
a Bulk-sequencing calls of KRAS variants of patient PR02’s 4 multiregional primary tumor samples. Venn diagram showing colocalization pattern of genetic variants TP53 p.C203Y, KRAS p.G12D, KRAS p.G12V in single nucleus of samples PR02-3 (b), PR02-4 (c). Single-cell genotype (HOM homozygous mutation, HET heterozygous mutation, WT wildtype) heatmap of samples PR02-3 (d), PR02-4 (e, zoomed in on 300 cells where tumor cells cluster). The KRAS p.G12D & p.G12V clone identities (above heatmaps) are identified as cells having “HET” or “HOM” genotype of each variant and are colored as labeled. Cells are hierarchically clustered based on the two KRAS variants’ single-cell AF. KRAS p.G12V clone size was 57 cells in PR02-3, 17 cells in PR02-4. f Representative H&E histology images of sample PR02-3. At least 3 representative pictures were taken per sample and yielded similar results.
