Fig. 5: SnDNA-seq identified two mutually exclusive SNV clones in a PDAC patient without KRAS mutation.
From: Application of high-throughput single-nucleus DNA sequencing in pancreatic cancer
a Representative H&E histology images of PDAC regions in samples PR01-1 (left, only the TGFBR2 double mutation was present), PR01-2 (right, only the FGFR1 mutation was present). At least 2 representative pictures were taken per sample and yielded similar results. b For sample PR01-3 (both the TGFBR2 double mutation and FGFR1 mutation were present), Venn diagram showing single-cell colocalization pattern of the TGFBR2 double mutation and FGFR1 mutation. c Single-cell genotype (HOM homozygous mutation, HET heterozygous mutation, WT wildtype) heatmap of 7 important variants pre-identified by bulk WES for sample PR01-3. Each cell’s clone identity (color strip above heatmap) is colored as shown by the figure legend. The FGFR1 and TGFBR2 SNV clones are defined as cells with non-wildtype genotype of each gene. The “putative normal” clone is defined as cells with “WT” genotype of all 7 genetic variants. Cells are hierarchically clustered within each clone. d Median per-amplicon ploidy of the TGFBR2 double mutation clone, the FGFR1 mutation clone and the putative normal clone found PR01-3. The putative normal clone is set as diploid baseline (green); amplicons with notable copy number loss and their corresponding genes are labeled. Source data are provided as a Source Data file.
