Fig. 6: SnDNA-seq revealed stepwise evolution in a metastatic PDAC case.
From: Application of high-throughput single-nucleus DNA sequencing in pancreatic cancer
a Single-cell genotyper (SCG)-inferred cluster information. Left panel: histogram showing each cluster’s size (number of nuclei) in the 3-sample cohort. Clusters are sorted based on size from top to bottom and assigned random colors to maximize heterogeneity. Middle panel: cluster CNV profile, represented by the homozygous deletion (homdel) status of 8 SMAD4 amplicons. Right: cluster SNV profile, represented by the genotype of 25 bulk-validated germline and somatic mutations. The 4 somatic mutations (identified by bulk sequencing on the same tumor sample) are colored red. b Cluster/clone phylogeny. Each cluster’s proportion in the cohort is labeled next to cluster ID. Each cluster-defining genomic event is labeled on its corresponding cluster. SNV events, as well as CNV events that affect them, are colored pink. c Cluster composition in each of the three multiregional autopsy samples of PA04. Technical replicates (fresh and frozen nuclei preparation) are merged. Source data are provided as a Source Data file.
