Fig. 3: Conserved mechanisms to achieve piRNA plasticity.
From: Amniotes co-opt intrinsic genetic instability to protect germ-line genome integrity
a Example of a duplication overlapping with a piRNA locus and its piRNA abundance from two chicken individuals. Blue represents Watson strand mapping reads; Red represents Crick strand mapping reads. Ppm, parts per million. b Example of an inversion overlapping with two piRNA loci (cluster 1047 and cluster 1195) along with their nonoverlapping control piRNA loci (cluster 542 and cluster 377) and their piRNA abundance from two chicken individuals. Blue represents Watson strand mapping reads; Red represents Crick strand mapping reads. Ppm, parts per million. c Example of a duplication overlapping with a piRNA locus. (Left) piRNA abundance from two chicken individuals. Blue represents Watson strand mapping reads; Red represents Crick strand mapping reads. Ppm, parts per million. (Right) piRNA species abundance from the two chicken individuals that have read counts of 1 to 9. d Box plots of piRNA variance of Abundance (left), Strand bias (middle), and Shannon diversity index (right) among 23 chickens from 6 breeds. Box plots show the 25th and 75th percentiles, whiskers represent the 5th and 95th percentiles, and midlines show median values. e Number of human pachytene piRNA loci (red) and randomly shuffled control sequences (aquamarine) overlapping with SV hotspots within de novo pathogenic SVs detected in patients (left), healthy human populations (middle), and historical SVs in the common ancestor of humans and great apes (right). Violin plots represent the medians of randomly shuffled control sequences that were computed 10,000 times.
