Fig. 3: In vivo screening of human MECR mutants in yeast. | Nature Communications

Fig. 3: In vivo screening of human MECR mutants in yeast.

From: An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration

Fig. 3

a Ten-fold dilution series testing for growth of wild-type and mutated human MECR variants on glucose (SCD-URA and YPD) and glycerol (SCG-URA) growth media. First row: Wild-type Bj1991α yeast strain with empty vector pYE352, Second row: Null mutant (Δetr1) Bj1991α yeast strain with empty vector pYE352, third row: Bj1991α Δetr1 yeast strain with mitochondrially targeted MECR in pYE352, fourth row to nineth row: Bj1991α Δetr1 with MECR single mutant in pYE352. b Western blot analysis of protein lipoylation and MECR expression in BJ1991α yeast expressing wild-type and mutated MECR variants. Actin and Ponceau staining were used as loading controls and the experiments were repeated three times with individual biological samples. Source data are provided as a Source data file.

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