Fig. 3: Metabolic labeling enriches different bacteria depending on the glycans applied. | Nature Communications

Fig. 3: Metabolic labeling enriches different bacteria depending on the glycans applied.

From: Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting

Fig. 3

a DESeq2 differential abundance analysis comparing the ESV abundance in the starting stool versus the glycan+ samples expressed as a volcano plot of the adjusted p value as a function of the log2-fold change. The horizontal line represents statistical significance and is drawn at an FDR of 0.1. The symbols are colored according to the phyla, and the ESVs that are statistically overrepresented in the glycan+ samples are labeled. b Distribution of the ESVs from the main phyla on the differential abundance axis. Statistical significance compared the Bacteroidetes and the Firmicutes by a two-tailed Mann‒Whitney test. c–f DESeq2 differential abundance bar graphs for the Bacteroidales order (c), Prevotella copri (d), Blautia wexlerae_1 (e), and Collinsella aerofaciens (f). Bar graphs represent log2 fold change ± log2 fold change standard error determined by DESeq2. Source data and statistical details are provided as a Source data file.

Back to article page