Fig. 5: Effect of nucleotide modification on base stacking energy of nucleotides.
From: High-throughput single-molecule quantification of individual base stacking energies in nucleic acids
a Modifications used in the study including 5′ Phosphorylated C, 5-methyl C, 5′ FAM modified C, 3′ ribose A. b Experimental data shows dissociation over time for constructs relative to their controls. c Off-rates observed for tethers with modified bases and their controls. d Free-energy of stacking calculated from off-rates in (c). Data in (b, c) presented as mean values +/− standard deviation from three independent data sets (shown in Supplementary Figs. 11–12). Data in (d) calculated from mean values +/− propagated error from (c). n represents the number of individual molecular tethers.
