Fig. 4: Specific histone acetyltransferases (HATs) are required for active INO1 localization. | Nature Communications

Fig. 4: Specific histone acetyltransferases (HATs) are required for active INO1 localization.

From: Adaptive partitioning of a gene locus to the nuclear envelope in Saccharomyces cerevisiae is driven by polymer-polymer phase separation

Fig. 4: Specific histone acetyltransferases (HATs) are required for active INO1 localization.The alternative text for this image may have been generated using AI.

a Schematic representation of the auxin-inducible degron (AID) system used to deplete targeted HAT catalytic subunits for degradation in the presence of auxin. b–g INO1 probability maps obtained from the analysis of thousands of nuclei in strains with different HATs tagged with the AID degron and grown in the absence of inositol (active). b–d HAT-AID tagged strains where there is no effect on the statistical distribution of the INO1 locus after treating the cells with auxin. e–g HAT-AID tagged strains where the addition of auxin results in disruption of the INO1 locus relocalization pattern to the nuclear periphery. h H3K14ac enrichment around and within the active INO1 gene, as determined by ChIP-qPCR and expressed as H3K14ac/H3 ratio, in the vicinity of DAS1, SNA3, INO1 and VPS35 gene loci for wild type and three different HAT-AID strains treated with auxin and under inositol activating conditions. i mRNA levels as quantified by RT-qPCR, relative to ACT1, for DAS1, SNA3, INO1 and VPS35 loci in wild type and Gcn5-AID, Taf1-AID, and Rtt109-AID strains in the presence of auxin and INO1 activating conditions. Source data are provided as a Source Data file. Data represent mean ± SD (h and i); n = 3 biological samples. Data were analyzed by unpaired two-sided Student’s t test and p-values are shown for significant differences between wild type (blue) and Gcn5-AID mutant (light purple). See also Supplementary Figs. 2–4.

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